The real-time polymerase chain reaction (RT-PCR) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It is used to amplify and simultaneously quantify a target sequence. For one or more specific sequences in a DNA sample, quantitative PCR enables both detection and quantification. The RT-PCR module manages and tracks this kind of experiments. A single experiment can include many samples analyzed for several amplicons. The module stores the results for each analyzed sample, using the LAS genomic annotation facility. The experiment description can be enriched with the raw data extracted by the instrument.
The user plans a set of aliquots for experiment using the Real Time PCR technology from the Biobank module. During the experiment planning the user defines also a title and description (optional). Before performing the molecular experiment, the user should validate the scheduled aliquots and confirm the used quantity for each aliquot. The user should insert the barcode of each aliquot to perform the validation procedure.
All experiments for which the samples have been validated are visualized. By selecting an experiment, the user can upload a file with the Real Time PCR results. A table with the measures is shown, according to the contents of the file. In addition to the measures, the user may also associate a set of files with an experiment.